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4ta Reunión Annual PulseNet América Latina Buenos Aires, Argentina June , 2006
Tânia Mara Ibelli Vaz
Experience of Instituto Adolfo Lutz, Central Public Health
Laboratory, São Paulo State, Brazil , on the use of standardized
Pulsed-Field Gel Electrophoresis - PulseNet Protocol
Instituto Adolfo Lutz São Paulo, Brazil
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BRASIL SÃO PAULO STATE
Instituto Adolfo Lutz *
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Government of São Paulo State
Secretary of Health, São Paulo
Coordination of Diseases Control CCD
Center of Epidemiological
Surveillance
Instituto
Adolfo Lutz
Division of Medical Biology
Dept.
Micro
Immunol
Section of Bacteriology
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Center of SanitarySurveillance
National Public Health Laboratories
NRLEC Regional Laboratories of Instituto Adolfo Lutz (11 Laboratories)
Private and Public Hospital Laboratories Paraná Santa Catarina RRL Central Public Health Laboratories Rio Grande do Sul Mato Grosso do Sul
Salmonella Shigella Vibrio cholerae
√ National Reference Laboratory for Escherichia coli (NRLEC)
Instituto Adolfo Lutz Central Public Health Laboratory São Paulo State, Brazil
√ Regional Reference Laboratory (RRL)
Strains to NRLEC / RRL sent by:
Characterization of Salmonella Enteritidis strains isolated from foodborneoutbreaks and from foods associated with these outbreaks
1,5,10 , molecular marker, S. Braenderup H9812; 2,3,6,7,8 and 9 , S. Enteritidis strains from humans;4, S. Enteritids from mayonnaise
1,5,10 – molecular marker S. Braenderup H9812 4,6,7,8 and 9, S.Enteritidis strains from humans; 2 and 3, S.Enteritidis from food
1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10
Fig.1A. XbaI PFGE patterns of S.Enteritidis strains
Fig 1B. XbaI PFGE patterns of S.Enteritidis strains
Characterization of Salmonella Johannesburg strains isolated from humans and foods
1 2 3 4 5 6 7 8 9 10
1,5,10 , molecular marker, Salmonella Braenderup H9812 2, 3 and 4, S. Johannesburg strains from humans; 6,7,8 and 9, S. Johannesburg strains from foods
Fig. 2. XbaI PGFE patterns of S. Johannesburg strains from humans and from foods
1 2 3 4 5 6 7 8
Salmonella Typhi strains isolated from two typhoid fever (TF) outbreaks and from sporadic cases of TF
1,8 , molecular, marker, S.Braenderup H9812; 2,3, S.Typhi from sporadic cases of TF; 4,5 STyphi from outbreak A; 6,7, S.Typhi from outbreak B
Fig.3. XbaI PFGE patterns of SalmonellaTyphi
Characterization of Salmonella I 1,4,[5],12:i:- and S.Typhimurium strains isolated from human and non- human sources (1991-2000)
1,8,15 – Molecular marker, S. Braenderup H9812 ( XbaI);2,3,4,5,6,7,9,10,11,12,13,14 , Salmonella I1,4,[5],12:i:-
Fig 4A. XbaI PFGE patterns of Salmonella I 1,4,[5],12:i:-
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Fig 4B. XbaI PFGE patterns of SalmonellaTyphimurium
1 2 3 4 5 6 7 8 9 10 11 1 13 14 15
1,8,15 – molecular marker S. Braenderup H9812 2,3,4,5,6,7,9,10,11,12,13,14 – S. Typhimurium
Characterization of Salmonella Typhimurium isolated from sporadic cases of human diseases and from non-human sources (2003-2004)
1,7,13 – molecular marker, S. Braenderup H9812 2,3,4,5,6,8,9,10,11,12 – S. Typhimurium strains
Fig.5 XbaI PFGE patterns of SalmonellaTyphimurium
1 2 3 4 5 6 7 8 9 10 11 12 13
1 2 3 4 5 6 7 8 9 10
Fig 6.XbaI PFGE patterns of E.coli O157:H7
1,5 and10- molecular marker; 2,3,4,6,7,8 and 9 – E.coli o157:H7 strains
Characterization of E.coli O157:H7
taibelli@ial.sp.gov.brvaztmi@uol.com.br
Tânia Mara Ibelli Vaz - VazTMIInstituto Adolfo Lutz Av.Dr.Arnaldo 351, 9o andarCEP 01246-902Cerqueira Cesar – São Paulo – SPBrazil
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