ANA 812

Anatomy & Neurobiology

ANA 812Digestive System III:

Liver, Gallbladder & Pancreas

Bethany CoxSarah MarksJoe SlusherKara Stebbins

Anatomy & Neurobiology

Slide #106 Liver- Pig 4x

Portal Area

Liver Lobule

Anatomy & Neurobiology

Slide #106 Liver- Pig

• Central Vein

10x

40xCentral Vein

• Cords of Hepatocytes• Liver Sinusoids• Binucleate Cell

Anatomy & Neurobiology

Slide #106 Liver- Pig4x40x

• Portal Vein• Hepatic Artery• Lymphatic Vessel• Bile Ductule

Anatomy & Neurobiology

Slide #106 Liver- Pig-40X

• Portal Vein• Lymphatic

• Bile Ductule

•Hepatic Artery

Anatomy & Neurobiology

Slide #101 Liver- Human-4X-Note relative lack of connectivetissue.

Anatomy & Neurobiology

Slide #101 Liver- Human-10X

•Central Vein

•Liver Sinusoids

•Hepatocyte Cords

•Lipid Droplets

Anatomy & Neurobiology

Slide #104 Liver- Mouse4x 40x

Portal VeinBile DuctuleLymphatic Vessel

Anatomy & Neurobiology

Liver: Von Kupffer cells-40X

H&E stain (human, slide 101)

Trypan blue stain with neutral red

(monkey, slide 103)Large oval nuclei projecting into sinusoids

Trypan blue phagocytized into granules

Anatomy & Neurobiology

Liver: One Effect of Cirrhosis-40X

Normal liver (H&E stain, human, slide 101)

Cirrhosis (Masson’s trichome stain,human, slide 107)

Vacuolated hepatocyte cytoplasm

Bile canaliculi

Anatomy & Neurobiology

Liver: Another Effect of Cirrhosis-4X

Normal liver (H&E stain, human, slide 101)

Cirrhosis (Masson’s trichome stain,human, slide 107)

Blue-staining collagen

(fewer hepatocytes, more CT)

Anatomy & Neurobiology

Slide #178 – Pancreas and Suprarenal Gland – Monkey

(Azan Stain) – 4X• Pancreatic

Islets of Langerhans (endocrine portion of the pancreas)

• Ducts– Intercalate

d– Intralobular– Interlobular

Anatomy & Neurobiology

Slide #178 – Pancreas and Suprarenal Gland – Monkey

(Azan Stain) – 40X• Islet of Langerhans

4X

Anatomy & Neurobiology

• More secretory acinar divided into lobules

• Ducts– Intercalated (not

seen at this power)– Intralobular– Interlobular

Slide #178 – Pancreas and Suprarenal Gland – Monkey

(Azan Stain) – 10X

Anatomy & Neurobiology

Slide #104-Gallbladder and liver-10X

•Simple columnar eplithelium

•Lamina propria

•Smooth muscle layer

•Definitive mucosal folds

Monkey, H&E stain

Anatomy & Neurobiology

Slide #105-Gallbladder -10X

•Human specimen after death

•Extremely folded gallbladder mucosa seen here is typical of human gallbadder

Human, H&E stain

Anatomy & Neurobiology

Comparison of Slides #104/#105-10X

Monkey, H&E stain

Human, H&E stain