2014-11-12 Oyster Presentation.pptx

8/10/2019 2014-11-12 Oyster Presentation.pptx

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Improvement of OysterBreeding in Malaysia

PrepaIFFAH INANI BT HASHIM (SE

NUR AINA AINI BT CHE MOHD SHUKRI (SE


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Introduction Oyster - any member of the families Ostreidae (true oysters) or

Aviculidae (pearl oysters)

Bivalve mollusks

Found in temperate and warm coastal waters of all oceans.

True oysters have been cultivated as food for more than 2,000 years


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Introduction

Species commercially found inMalaysia: Crassostrea belcheri Crassostrea iredalei Ostrea folium Saccostrea spp.

SPECIES

Crassostrea belcheri

Johore

Sabah

Sarawa

Crassostrea rivularis Sarawa

Saccostrea cucullataSabah

Sarawa

Ostrea folium Kedah


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Life cycle Reproductive prime - at about 3 years

of age. Once adult oysters are ripe they can

begin to spawn. After fertilization drift in water

cell division become juvenilelarvae

After two weeks old concentrateat the bottom of the river attachedto hard substrate undergocomplete metamorphosis becomespat

Become juvenile at one year become adult at 3 year old.


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C r as s o s t r e a b e l c h er i

Chromosome number, (n=10) Potential for aquaculture High demand by farmers compared with other local oyster species High demand among customers


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Problem statement

Low growth rate

Expose to: Siltation (Water

pollution) Predation by xanthid

crab and starfish Poor spatfall

Result in: Low yield Lack of na

seed

Oyster farming can be a lucrative bucan fetch RM5 per piece if sold based

price

-Borneo Post, 2013


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Goals

To improve growth rate of local C. belcheri oyster To produce oysters seeds in term of quality and quantity Method: Crossbreeding

Between local C. belcheri and fast growth C. belcheri from Thailand

C. belcheri Malaysia Thailand

Size (Average) 12-14 cm 10-15 cm

Growth rate > 1 year 7-8 month

Description Problem in sustaining theindustry due to insufficientseed stock

Able to export its oyters(ahead from other ASEANcountries)


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Breeding Design (crossbreed)

C. belcheri (Malaysia) X C. belcheri (Thailand)

F1(selection for fast grow progeny)

(F1XF1 self cross)

Stable (superior breeds)


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Experimental DesignCrossbreed between C. Belcheri from Malaysia andThailand

(C. Belcheri (Malaysia) X C. belcheri (Thailand) )

Monitor the F1 generation: Looking at growth Compare the growth rate between the offspring

After 4 or 5 month, rank the offspring based on the fastgrowth to later growth

Once has ranked the offspring, select for the fastestgrowth and normal growth: Laboratory work: RNA extraction and send for

sequencing

Phen


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Sequencing result: Compare both sequence fast grow and normal Choose growth gene in osyter such as amylase gene Compare the gene in the both sequence To see any mutation occur in the fast grow offspring

If there are mutation occurs in the fast grow progeny (e.g SNP) Hypothesis: The fast growth osyter is because of the

mutation occur in the growth gene

Design primer for the marker To screen for fast grow osyter


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Conclusion


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References

The 4th International Oyster Symposium (IOS4) . (2011). Prudence, M., Moal, J., Boudry, P., Daniel, J. Y., Quere, C., Jeffroy, F

. Huvet, A. (2006). An amylase gene polymorphism is associated wgrowth differences in the Pacific cupped oyster Crassostrea gigas.

Animal Genetics . doi:10.1111/j.1365-2052.2006.01481.x


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Documents

2014-11-12 Oyster Presentation.pptx