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8/16/2019 Dinesh Dissertation Presentation
http://slidepdf.com/reader/full/dinesh-dissertation-presentation 1/17
Presentation submitted
by
Dinesh Rathva
CUG/2014/1059
Under the guidance o
Pro! "an #ingh
Dean
#choo$ o chemica$ #ciences
Centra$ University o Gu%arat& Gandhinagar
'(('C) *( #UR(+C)+,)# *, P+R)-)-*,
C*'((-C-',) *( -*D-,' -, ).* P+#'
#*',) ##)'"
8/16/2019 Dinesh Dissertation Presentation
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Contents of my Dissertation
Prob$em )heme
-ntroduction
*b%ective
"ateria$ and methods
Resu$t and Discussion
Conc$usion
(uture Pros3ect
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Prob$em )heme
•
-odine deiciency causes goiter
• Radioactive iodine stabi$ied by stabi$ier& hich can strong$y aect the target
organ in the body
• -n meta$$urgy& the 3artition coeicient is an im3ortant actor in determining ho
dierent im3urities are distributed beteen mo$ten and so$idiied meta$
• + drug6s 3artition coeicient #trong$y aects7
the drug intended target
its eect once it reaches its target
time 3eriod o its activity
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-ntroduction
• Partition coeicient 8 ratio o concentrations o a com3ound in a miture o to
immiscib$e 3hases at e:ui$ibrium $i;e a:ueous and organic
• Partition coeicient o substance can be deined as7 <3conc! * a:! Phase / Conc!
* organic 3hase
• Partition coeicients are useu$ in estimating the distribution coeicient o drugs
ithin the body
• -mmiscib$e so$vents 3hases have been used as a uni:ue mode$ or study o 3artition
coeicient
• )he mutua$ miscibi$ity is obtained by adding the suractants to a set o immiscib$e
so$vents that cause hydro3hi$ic and hydro3hobic interactions
• )he suractant decrease the chemica$ 3otentia$ o individua$ so$vent due to
interaction as 3er $i:uid8$i:uid interacia$ mode$ o so$vents
•
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*b%ectives o my study
• Determination o 3artition coeicient o iodine conc! in a:ueous /organic 3hase
• Determination o concentration iodine absorbed in a:! suractants ith organic 3hase
• Determination o 3artition entro3y and Gibbs ree energy by using =o$tmann
distribution $a
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Materials: -odine>-2? & CC$4& )een 20& )een 40 &)een @0& )een A0& #tarch >indicator?& #odium
thiosu$3hate >,a2#2*B!52*? and "i$$i3ore ater
Method:
• 0!04" iodine as 3re3ared in CC$4
• 0!005" non8ionic suractants ere se3arate$y 3re3ared in 50m ater7)een820& )een840& )een8@0
and )een8A0
• )he chromatogra3hic se3aration method as used or determine 3artition coeicient o -2 in ater and
a:! #uractants!
• (irst$y& =y ta;ing 20m o -2 so$ution in se3arating unne$ and then 40m ater added in se3arating
unne$ and sha;e and ;e3t or e:ui$ibrium! +ter that to 3hase overcome >a:ueous 3hase and organic
3hase?& se3arate them and titration o both 3hase ere carried out ith 0!01" sodium thiosu$3hate by
using starch as an indicator
• +nd simi$ar method ere used or a:! suractants to determine P!C!
Characterization methods
Dynamic $ight #cattering to determine dis3ersity o iodine in a:! Phases
U8isib$e #3ectrosco3y )o determine absorbance o -odine in res3ective +:! Phases
"ateria$s "ethod
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Separation method for Kp determination
After Shaking
Aq. Phase
Org. Phase
Separation ofphases
Take Aq. Layer from each ask and titrate ith sodi!mthios!" hate
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Distribution Pattern o -odine in +:! Phases
I2 in
water
I2 inCCl4
I2 inTween 20
I2 inTween 40
Conti####
Resu$ts Discussion
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Conti####
I2 inTween 60
I2 inTween 80
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+bsorbance o -2 in *rganic Phase
200 B00 400 500 @00 E00 A00
0
0!5
1
1!5
2
2!5
B
B!5
4
4!5
I2 in CCL4
.ave$ength >nm?
+bsorbance
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Partition coeicient o -odine<3F >Conc! o -2 in a:! Phase / Conc! o -2 in *rg! Phase?
$irstorder
%.%%%% %.%%&% %.%%'% %.%%(% %.%%)% %.%%*% %.%%+% %.%%,% %.%%-% %.%%% %.%&%%
%.%%
%.%*
%.&%
%.&*
%.'%
%.'*
%.(%
%.(*
f/01 2 ((.0 3 %.%&
45 2 &
Concentration of Aq. Phases
Kp
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(ree energy o iodine in +:! Phases
Aq. Phases Conc.of aq. Phase 67
8ater %.%%& (,,).)
T3'% %.%%' (%'-.,)
T3)% %.%%( ',&.%)
T3+% %.%%( '*'.')
T3-% %.%% &'+.&'
$irst order
%.%%% %.%%& %.%%' %.%%( %.%%) %.%%* %.%%+ %.%%, %.%%- %.%% %.%&%
%
*%%
&%%%
&*%%
'%%%
'*%%
(%%%
(*%%
)%%%
45 2 %.(
Concentration of Aq. Phase
Free energy !"#ol$
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Conc$usion
• )een 20 to )een A0 -odine goes rom CC$4 to 2*
• Dis3ersion o iodine changed ith varying )eens series rom )820 to
)8A0!
• +bsorbance o -odine observed in U range >2008400nm? not in isib$e
range >400 A00 nm?!
• (ree energy o iodine in a:! Phase decrease ith increasing
concentration o iodine!
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(uture Pros3ect
• +t - suractant gets de3osit
• -odine so$ubi$ity increasing in a:ueous 3hase
• #uractant contro$ on -odine distribution
• -n 3$ace CC$4& D"#*& D"'& 'ther and benene ou$d be
used
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+c;no$edgements
%y &'per(isor Prof. %an &ingh) *ean &C&) C+,
*r. P.C. !ha) *r. *hanan-ay %onal) *r. &#ritile/ha era) *r. 1.a-' Cha'han
%r. harat . atho
Friens
Fa#ily #e#3ers