13
HCG uman Chorionic Gonadotropi Dwi Amalia Yuni Sulistiani Anisa Rahmadini Denny Agung Nugroho D3 ANALIS KESEHATAN STIKES JENDERAL ACHMAD YANI CIMAHI

Presentation1 imun

Embed Size (px)

DESCRIPTION

tugas

Citation preview

Page 1: Presentation1 imun

HCG (Human Chorionic Gonadotropin)

Dwi AmaliaYuni Sulistiani

Anisa RahmadiniDenny Agung NugrohoD3 ANALIS KESEHATAN

STIKES JENDERAL ACHMAD YANI CIMAHI

Page 2: Presentation1 imun

TEORITICAL BACKGROUND

HCG (Human Chorionic Gonadotropin) is a hormone produced by placenta tissue in

early pregnancy. This hormone will be released through the urine.

Page 3: Presentation1 imun

PURPOSE

To detect the presence of human chronic gonadotropin in urine and also detects the presence of pregnancy

Page 4: Presentation1 imun

The direct pregnancy assay is based upon the agglutination reaction between latex particles coated with anti-hCG antibodies and hCG present in the test specimen.

PRINCIPLE

Page 5: Presentation1 imun

Tools and Material

Used to protecting the body

Lab Coat Mask

Used to protect nose and mouth

Page 6: Presentation1 imun

Gloves Slide test

Used to protect hand from infection subtance

Used to interpretation the result

Page 7: Presentation1 imun

microtube pippette

It can used to pippette and stirring

Used to Place the sample

Page 8: Presentation1 imun

Lateks reagent Disinfectant

Used to detect antigen in sampel

Used to sterilization pippette & stirring

Page 9: Presentation1 imun

Control (-)

Used to standard control (-) for sample

Control (+)

Used to standard control (+) for sample

Page 10: Presentation1 imun

Procedure • Bring the reagent and spesimen to room temperature before use• Place one drop of the negative pregnancy control onto a circle of the

agglutination slide• Using the pippet-stirrers provided, place one drop of the urine specimen

onto the remaining circle of the agglutination slide• Shake and re-suspend the pregnancy latex reagent. Add one drop to each

of the test circles of the agglutination slide• Stir with individual pippete-stirrer and spread mixture over entire area of

the test circle• Gently rock the agglutination test slide for two minutes and observe the

test circles for agglutination. Interpret results at two minutes. Extended incubation may result in evaporation and erroneous result

Page 11: Presentation1 imun

Interpretation of results

• Positive result : agglutination occurs within two minutes

• Negative result : agglutination does not occur within two minutes

Page 12: Presentation1 imun

RESULT

• In urine Mrs. .... Negative pregnancy because there’s not occur agglutination. This mean Mrs. ... Is not pregnancy and of course there is in urine not contain antigen anti-HCG.

Page 13: Presentation1 imun

Thank you