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Polymer Statistics III Mixtures of polymers applications to proteins and nucleic acids

Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

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Page 1: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

Polymer Statistics III Mixtures of polymers

applications to proteins and nucleic acids

Page 2: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

recap: scaling exponents and coil-to-globule

Page 3: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

-Tltr(Lt1p»lttLrtics

rt ix iÀG

Cotsisr oT fdo oQ iadtr biffieaatq- ctenìcAlHilrru n-l5?eei€3 .

tfruEmìXnnrìsu^JìroonAd»Aucsn?oilarrSA/tEil-rgnnixaD o rJ ft flo urctt*tl go*(E r rtlf- nì xa11ttÉ i s

A,urcÈ +lono Gl:-rnlrrlos Ix&n?G.: A ?au(nAt ;d fuo» bul€§T

lf rrt€ nixrr)ttf Ca'a'i579 o; *e:Ùer?AL DtFrett?^'y; ÈtAges

( nrAoSS W;--' tl » irevr'alt cen?eQ'no"r:) ' i' i 5 64 aiaN

+leT€le6gzJeouS IoZ*n?É, i oit- AÀb \^'ArElt

Page 4: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

-Tltr(Lt1p»lttLrtics

rt ix iÀG

Cotsisr oT fdo oQ iadtr biffieaatq- ctenìcAlHilrru n-l5?eei€3 .

tfruEmìXnnrìsu^JìroonAd»Aucsn?oilarrSA/tEil-rgnnixaD o rJ ft flo urctt*tl go*(E r rtlf- nì xa11ttÉ i s

A,urcÈ +lono Gl:-rnlrrlos Ix&n?G.: A ?au(nAt ;d fuo» bul€§T

lf rrt€ nixrr)ttf Ca'a'i579 o; *e:Ùer?AL DtFrett?^'y; ÈtAges

( nrAoSS W;--' tl » irevr'alt cen?eQ'no"r:) ' i' i 5 64 aiaN

+leT€le6gzJeouS IoZ*n?É, i oit- AÀb \^'ArElt

Page 5: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

-Tltr(Lt1p»lttLrtics

rt ix iÀG

Cotsisr oT fdo oQ iadtr biffieaatq- ctenìcAlHilrru n-l5?eei€3 .

tfruEmìXnnrìsu^JìroonAd»Aucsn?oilarrSA/tEil-rgnnixaD o rJ ft flo urctt*tl go*(E r rtlf- nì xa11ttÉ i s

A,urcÈ +lono Gl:-rnlrrlos Ix&n?G.: A ?au(nAt ;d fuo» bul€§T

lf rrt€ nixrr)ttf Ca'a'i579 o; *e:Ùer?AL DtFrett?^'y; ÈtAges

( nrAoSS W;--' tl » irevr'alt cen?eQ'no"r:) ' i' i 5 64 aiaN

+leT€le6gzJeouS IoZ*n?É, i oit- AÀb \^'ArElt

Page 6: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

nivi,J G (l

, Trn}o $?€a'€S A A^)§ B

.dovoLùlÉctwùG€t/+tedniyr^}é.rl(§Con?olg7ff

V6 Vo -+ Va+Vs

, V oLùrW r(wcrt'oi ;

rV*VaP=-=Tft Vror Va + Vs Vtrt V* + Vg

Qr: 4 - (nuSu AU-Y , dt Ql?fiz§-- T+t6 yixrt)n€ oi A (41Tì 6 '

w, Ass orl, t*AT hw auz c-orlpo^JÉa,rs a^ tsa xscABrD

lùT.gonsoflEuenglrilL,(.,lrlìrsoTTft6t*nie,To rL €KqIr€ ts .'/ c+loo<''Jc * wtr c€ tÀv€ {t€ €̂ Étf

urJ ri- +{A s aala uoL ÙY1€ oF A ltoJ snrt2* aT O lK

(auYnweY, @

Page 7: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

nivi,J G (l

, Trn}o $?€a'€S A A^)§ B

.dovoLùlÉctwùG€t/+tedniyr^}é.rl(§Con?olg7ff

V6 Vo -+ Va+Vs

, V oLùrW r(wcrt'oi ;

rV*VaP=-=Tft Vror Va + Vs Vtrt V* + Vg

Qr: 4 - (nuSu AU-Y , dt Ql?fiz§-- T+t6 yixrt)n€ oi A (41Tì 6 '

w, Ass orl, t*AT hw auz c-orlpo^JÉa,rs a^ tsa xscABrD

lùT.gonsoflEuenglrilL,(.,lrlìrsoTTft6t*nie,To rL €KqIr€ ts .'/ c+loo<''Jc * wtr c€ tÀv€ {t€ €̂ Étf

urJ ri- +{A s aala uoL ÙY1€ oF A ltoJ snrt2* aT O lK

(auYnweY, @

*

Page 8: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

nivi,J G (l

, Trn}o $?€a'€S A A^)§ B

.dovoLùlÉctwùG€t/+tedniyr^}é.rl(§Con?olg7ff

V6 Vo -+ Va+Vs

, V oLùrW r(wcrt'oi ;

rV*VaP=-=Tft Vror Va + Vs Vtrt V* + Vg

Qr: 4 - (nuSu AU-Y , dt Ql?fiz§-- T+t6 yixrt)n€ oi A (41Tì 6 '

w, Ass orl, t*AT hw auz c-orlpo^JÉa,rs a^ tsa xscABrD

lùT.gonsoflEuenglrilL,(.,lrlìrsoTTft6t*nie,To rL €KqIr€ ts .'/ c+loo<''Jc * wtr c€ tÀv€ {t€ €̂ Étf

urJ ri- +{A s aala uoL ÙY1€ oF A ltoJ snrt2* aT O lK

(auYnweY, @

*

Page 9: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

nivi,J G (l

, Trn}o $?€a'€S A A^)§ B

.dovoLùlÉctwùG€t/+tedniyr^}é.rl(§Con?olg7ff

V6 Vo -+ Va+Vs

, V oLùrW r(wcrt'oi ;

rV*VaP=-=Tft Vror Va + Vs Vtrt V* + Vg

Qr: 4 - (nuSu AU-Y , dt Ql?fiz§-- T+t6 yixrt)n€ oi A (41Tì 6 '

w, Ass orl, t*AT hw auz c-orlpo^JÉa,rs a^ tsa xscABrD

lùT.gonsoflEuenglrilL,(.,lrlìrsoTTft6t*nie,To rL €KqIr€ ts .'/ c+loo<''Jc * wtr c€ tÀv€ {t€ €̂ Étf

urJ ri- +{A s aala uoL ÙY1€ oF A ltoJ snrt2* aT O lK

(auYnweY, @

nivi,J G (l

, Trn}o $?€a'€S A A^)§ B

.dovoLùlÉctwùG€t/+tedniyr^}é.rl(§Con?olg7ff

V6 Vo -+ Va+Vs

, V oLùrW r(wcrt'oi ;

rV*VaP=-=Tft Vror Va + Vs Vtrt V* + Vg

Qr: 4 - (nuSu AU-Y , dt Ql?fiz§-- T+t6 yixrt)n€ oi A (41Tì 6 '

w, Ass orl, t*AT hw auz c-orlpo^JÉa,rs a^ tsa xscABrD

lùT.gonsoflEuenglrilL,(.,lrlìrsoTTft6t*nie,To rL €KqIr€ ts .'/ c+loo<''Jc * wtr c€ tÀv€ {t€ €̂ Étf

urJ ri- +{A s aala uoL ÙY1€ oF A ltoJ snrt2* aT O lK

(auYnweY, @

b

Page 10: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

Degree of polymerization

Page 11: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

----|.e-^t-, nrrv [, 5= (a 0d -Q-ò \

^rn'ce ofoaàùlt*noJs

/Vl = ru0rtBuR. a€ LfrTiC€ =ìtz:-NO, Ng : D?6nE€ or ?ocvnaz^?4r,o^J

. lnl A qono &,t(n1g mtrrr.lnr 6 ft Adb 6 eActt ttotzci(E

H[S

-Q-Ag = lw

' a$é u?€è;4 À b#p1 n ixrJ6 ('* A ?ani "A' "^,=)

-§LA = rnf.

, Tt(f c+tA,JW ì^l gr'rrno?( is &'Ùe?ù Bt :

AS^ = sJ* rcrid& sf* niri"rc -

= Ks \-Q*o Kg 4-0+ =

=&!dLr ò?*AcuA'rS losiiwA*<L

= Kb -L\$r = Ka lq Étr ò _rLA 'r Ò /40

Page 12: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

----|.e-^t-, nrrv [, 5= (a 0d -Q-ò \

^rn'ce ofoaàùlt*noJs

/Vl = ru0rtBuR. a€ LfrTiC€ =ìtz:-NO, Ng : D?6nE€ or ?ocvnaz^?4r,o^J

. lnl A qono &,t(n1g mtrrr.lnr 6 ft Adb 6 eActt ttotzci(E

H[S

-Q-Ag = lw

' a$é u?€è;4 À b#p1 n ixrJ6 ('* A ?ani "A' "^,=)

-§LA = rnf.

, Tt(f c+tA,JW ì^l gr'rrno?( is &'Ùe?ù Bt :

AS^ = sJ* rcrid& sf* niri"rc -

= Ks \-Q*o Kg 4-0+ =

=&!dLr ò?*AcuA'rS losiiwA*<L

= Kb -L\$r = Ka lq Étr ò _rLA 'r Ò /40

Page 13: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

----|.e-^t-, nrrv [, 5= (a 0d -Q-ò \

^rn'ce ofoaàùlt*noJs

/Vl = ru0rtBuR. a€ LfrTiC€ =ìtz:-NO, Ng : D?6nE€ or ?ocvnaz^?4r,o^J

. lnl A qono &,t(n1g mtrrr.lnr 6 ft Adb 6 eActt ttotzci(E

H[S

-Q-Ag = lw

' a$é u?€è;4 À b#p1 n ixrJ6 ('* A ?ani "A' "^,=)

-§LA = rnf.

, Tt(f c+tA,JW ì^l gr'rrno?( is &'Ùe?ù Bt :

AS^ = sJ* rcrid& sf* niri"rc -

= Ks \-Q*o Kg 4-0+ =

=&!dLr ò?*AcuA'rS losiiwA*<L

= Kb -L\$r = Ka lq Étr ò _rLA 'r Ò /40

Page 14: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

----|.e-^t-, nrrv [, 5= (a 0d -Q-ò \

^rn'ce ofoaàùlt*noJs

/Vl = ru0rtBuR. a€ LfrTiC€ =ìtz:-NO, Ng : D?6nE€ or ?ocvnaz^?4r,o^J

. lnl A qono &,t(n1g mtrrr.lnr 6 ft Adb 6 eActt ttotzci(E

H[S

-Q-Ag = lw

' a$é u?€è;4 À b#p1 n ixrJ6 ('* A ?ani "A' "^,=)

-§LA = rnf.

, Tt(f c+tA,JW ì^l gr'rrno?( is &'Ùe?ù Bt :

AS^ = sJ* rcrid& sf* niri"rc -

= Ks \-Q*o Kg 4-0+ =

=&!dLr ò?*AcuA'rS losiiwA*<L

= Kb -L\$r = Ka lq Étr ò _rLA 'r Ò /40

Page 15: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

ASn^^ixm

= /YtA ASo r

= € K6

YAS,=/\

lù-l-L nro

44r+ k4l{Ng

fdor*uuS.}pdrto")S

c{4Ad(rS

, ?aLL( r:a.rL

AlmoP< foftbulrio"lS / AÀ»

QdCrÙtA,tL

(otvnZrz Ègl»S-

O

per lattice site

total entropy

Page 16: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

ASn^^ixm

= /YtA ASo r

= € K6

YAS,=/\

lù-l-L nro

44r+ k4l{Ng

fdor*uuS.}pdrto")S

c{4Ad(rS

, ?aLL( r:a.rL

AlmoP< foftbulrio"lS / AÀ»

QdCrÙtA,tL

(otvnZrz Ègl»S-

O

ASn^^ixm

= /YtA ASo r

= € K6

YAS,=/\

lù-l-L nro

44r+ k4l{Ng

fdor*uuS.}pdrto")S

c{4Ad(rS

, ?aLL( r:a.rL

AlmoP< foftbulrio"lS / AÀ»

QdCrÙtA,tL

(otvnZrz Ègl»S-

O

Page 17: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

Ingredients:-  Five10-ballblackchains-  Five10-ballwhitechains

Trytoconnectthewhitebeadsandformthefive10-ballwhitechains…

Filltheschemewithfive10-ballblackAndfive10-ballwhitechains…

Rules:-NointersecDonallowed-Onebeadbelongstoonesinglechain

Page 18: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

lNrdWAa,JS

I euertcy or rzi fi^l c

SPeq'es A A^ìt B

?AiLd i}t i "venx64'f o à S

A+{< 141744c7oJ e,ìafzb( oÈ

'----= or -rt{€ S(Ec;e5 AA \tr^t42zt,.,y.gJ o s- o,l rft-r ?aogeyt'drT

oF ÉdNJ I F t À" oa .È "4s

ila6l eao45

U+ 7 Uqn 4^t Uog 4e

Un* )\)so \ 3Uoe ) +

.?fu

U6 = t)eeh + rJeB 4,

{HiS iS 1+1€. tlr€n+qioiB€np€E{ flo Fro.òorzzrc5 ,

l^l ft W'ff (f -tllztrtz AL;nuLn ?tE Po53i Btz xÉfuPotsA T THz sA rtr dr1-? '

T+L€ *r/efW C< t'JreYl*a:9n)r'§ e U* rdìr*r ?getÀ0 tW C@fU\lAaoJNonbetL

Page 19: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

lNrdWAa,JS

I euertcy or rzi fi^l c

SPeq'es A A^ìt B

?AiLd i}t i "venx64'f o à S

A+{< 141744c7oJ e,ìafzb( oÈ

'----= or -rt{€ S(Ec;e5 AA \tr^t42zt,.,y.gJ o s- o,l rft-r ?aogeyt'drT

oF ÉdNJ I F t À" oa .È "4s

ila6l eao45

U+ 7 Uqn 4^t Uog 4e

Un* )\)so \ 3Uoe ) +

.?fu

U6 = t)eeh + rJeB 4,

{HiS iS 1+1€. tlr€n+qioiB€np€E{ flo Fro.òorzzrc5 ,

l^l ft W'ff (f -tllztrtz AL;nuLn ?tE Po53i Btz xÉfuPotsA T THz sA rtr dr1-? '

T+L€ *r/efW C< t'JreYl*a:9n)r'§ e U* rdìr*r ?getÀ0 tW C@fU\lAaoJNonbetL

i,r-l{ts

Au-n =

C+tAà6€-

?- U,/Y1

Eò}ÉzdY oJ rti xir..) 6 is tttt

$+ (^-d) ('u^u-unft -'ru')

(? a*s - UnR - Uee)7L Kor

t-_lftttr i ^trettqciias?Atut€Iw-

44,'* =

AU-.ix

AS-ix, -rx

tuN* *.%(4*)rv(44-\

AI-,* : A U*,* - T- AS prit =

=Ksr[k%q^+ *%0 4^).' r4*(a-4^l

r?nfi,t Kry1 ,i'q*[ $r-rl_t ?",ì-1 -1'T'l

^#U' tiqi

[, * r,' u'J

'l i; \l \,' u'\r' J \l'

Page 20: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

lNrdWAa,JS

I euertcy or rzi fi^l c

SPeq'es A A^ìt B

?AiLd i}t i "venx64'f o à S

A+{< 141744c7oJ e,ìafzb( oÈ

'----= or -rt{€ S(Ec;e5 AA \tr^t42zt,.,y.gJ o s- o,l rft-r ?aogeyt'drT

oF ÉdNJ I F t À" oa .È "4s

ila6l eao45

U+ 7 Uqn 4^t Uog 4e

Un* )\)so \ 3Uoe ) +

.?fu

U6 = t)eeh + rJeB 4,

{HiS iS 1+1€. tlr€n+qioiB€np€E{ flo Fro.òorzzrc5 ,

l^l ft W'ff (f -tllztrtz AL;nuLn ?tE Po53i Btz xÉfuPotsA T THz sA rtr dr1-? '

T+L€ *r/efW C< t'JreYl*a:9n)r'§ e U* rdìr*r ?getÀ0 tW C@fU\lAaoJNonbetL

i,r-l{ts

Au-n =

C+tAà6€-

?- U,/Y1

Eò}ÉzdY oJ rti xir..) 6 is tttt

$+ (^-d) ('u^u-unft -'ru')

(? a*s - UnR - Uee)7L Kor

t-_lftttr i ^trettqciias?Atut€Iw-

44,'* =

AU-.ix

AS-ix, -rx

tuN* *.%(4*)rv(44-\

AI-,* : A U*,* - T- AS prit =

=Ksr[k%q^+ *%0 4^).' r4*(a-4^l

r?nfi,t Kry1 ,i'q*[ $r-rl_t ?",ì-1 -1'T'l

^#U' tiqi

[, * r,' u'J

'l i; \l \,' u'\r' J \l'

tptticzz»

L}lft'cz.3D

4

6

Z = C@Ql'»'el*Tr oJ lAnoer\

Arf€rrloa r.;,Jerur ftaL noÀotrc7- i 5a,LUÌ= L U*

L<Ùs7 = L dgL

fttr TorAL @€rlc'.(

u =?60 u*2\

W Affr.n rr"trd6

ni Xrit,l& lS:

\+ (vts 0.) =

L*Luas 4r'4-{ dea 6:) \

fJ- Z uou +r (r-+t*us6(, +l)U(t''** 4r

T ('-^T +te To-r &L E»€rU'{ ÈetuQi

O= 9g uas lnYoA LUoa = TtL, Ò ut QsJL

(*^#u*ù

A z=coordination number

Page 21: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

lNrdWAa,JS

I euertcy or rzi fi^l c

SPeq'es A A^ìt B

?AiLd i}t i "venx64'f o à S

A+{< 141744c7oJ e,ìafzb( oÈ

'----= or -rt{€ S(Ec;e5 AA \tr^t42zt,.,y.gJ o s- o,l rft-r ?aogeyt'drT

oF ÉdNJ I F t À" oa .È "4s

ila6l eao45

U+ 7 Uqn 4^t Uog 4e

Un* )\)so \ 3Uoe ) +

.?fu

U6 = t)eeh + rJeB 4,

{HiS iS 1+1€. tlr€n+qioiB€np€E{ flo Fro.òorzzrc5 ,

l^l ft W'ff (f -tllztrtz AL;nuLn ?tE Po53i Btz xÉfuPotsA T THz sA rtr dr1-? '

T+L€ *r/efW C< t'JreYl*a:9n)r'§ e U* rdìr*r ?getÀ0 tW C@fU\lAaoJNonbetL

i,r-l{ts

Au-n =

C+tAà6€-

?- U,/Y1

Eò}ÉzdY oJ rti xir..) 6 is tttt

$+ (^-d) ('u^u-unft -'ru')

(? a*s - UnR - Uee)7L Kor

t-_lftttr i ^trettqciias?Atut€Iw-

44,'* =

AU-.ix

AS-ix, -rx

tuN* *.%(4*)rv(44-\

AI-,* : A U*,* - T- AS prit =

=Ksr[k%q^+ *%0 4^).' r4*(a-4^l

r?nfi,t Kry1 ,i'q*[ $r-rl_t ?",ì-1 -1'T'l

^#U' tiqi

[, * r,' u'J

'l i; \l \,' u'\r' J \l'

tptticzz»

L}lft'cz.3D

4

6

Z = C@Ql'»'el*Tr oJ lAnoer\

Arf€rrloa r.;,Jerur ftaL noÀotrc7- i 5a,LUÌ= L U*

L<Ùs7 = L dgL

fttr TorAL @€rlc'.(

u =?60 u*2\

W Affr.n rr"trd6

ni Xrit,l& lS:

\+ (vts 0.) =

L*Luas 4r'4-{ dea 6:) \

fJ- Z uou +r (r-+t*us6(, +l)U(t''** 4r

T ('-^T +te To-r &L E»€rU'{ ÈetuQi

O= 9g uas lnYoA LUoa = TtL, Ò ut QsJL

(*^#u*ù

A z=coordination number

i,r-l{ts

Au-n =

C+tAà6€-

?- U,/Y1

Eò}ÉzdY oJ rti xir..) 6 is tttt

$+ (^-d) ('u^u-unft -'ru')

(? a*s - UnR - Uee)7L Kor

t-_lftttr i ^trettqciias?Atut€Iw-

44,'* =

AU-.ix

AS-ix, -rx

tuN* *.%(4*)rv(44-\

AI-,* : A U*,* - T- AS prit =

=Ksr[k%q^+ *%0 4^).' r4*(a-4^l

r?nfi,t Kry1 ,i'q*[ $r-rl_t ?",ì-1 -1'T'l

^#U' tiqi

[, * r,' u'J

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Page 22: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

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Page 24: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

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Page 25: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

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Page 27: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

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Page 28: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

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Page 29: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

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Page 32: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

PEG200

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Page 33: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

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Page 34: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

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Page 35: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

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Page 36: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3
Page 37: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

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Page 38: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

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Page 39: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

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Page 40: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

intracellular phase-separation

Page 41: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3
Page 42: Structure and Properties of Nucleic Acid Components5' 3' 3' 5' DNA Replication: 5' to 3', template directed via Watson Crick Base Pairing DNA Polymerase . O G A C T A C T G 5' 5' 3

WHAT HAVE WE LEARNED?

Ideal polymers behawe as a drunk (random valk!)

Styrofoam does not soak in coffee because

coffee is a poor solwent for polystyrene

(coil to globule)

Polymer mixtures can phase-separates,

forming droplets but also hydrogels

(contact lenses)