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Investigacin fundacional, Pilar de la Innovacin tecnolgica
Fernando Lpez-Daz, Ph.D., CBL.
Staff Scientist, The Salk Institute for Biological Studies, La Jolla, USA Adjunct Professor Master in Advanced Therapies, UFV, Madrid, SPAIN
Molecular Tumor Board, UCSD Moores Cancer Center, La Jolla, USA Intellectics Inc. San Diego, USA ; CEPIMP Genomics SA, ARGENTINA
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Medicina Genmica
Estrategias genmicas rutinarias en la oncologa clnica
>20-40%tumor
TumorBiopsy
1.DNA/RNASequencing2.Anlisiscien
1. Guia teraputica 2. Investigacin aplicada y clinica -Clinical Trial n=1 3. Drug Discovery. 4. Vigilancia genmica
Pathways Analysis
Incidence
A novel ESR1 mutation from a metastatic prostate carcinoma tumor
Structure Analysis
Laretroalimentacindelconocimientoenbiomedicina:ClinicalPracOceandBiomedicalResearch
Target validation
Drug discovery
Clinical trials
Clinical Practice
Target discovery
7
8
DNA damage
Apoptosis
Pre-malignant cells Primary tumor
>Mutations & selection
Early dissemination
Late dissemination
Blood vessels
Metastases
Normal cells
Cancer Development, Progression and Cellular Stress
Adapted from Klein C., Science 2008
development progression
10
2013 Target Discovery:
Technology Dev. & Concept Discovery:
Cancer evolution and Drug Resistance 2014
2013: Target discovery: Control of the stress response in precancerous and cancer cells
2016: using CRISPR technology to produce a assay platform for new compounds screen
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2014: SINGLE CELL RNA SEQUENCING: SELECTIVE / ADAPTIVE EVOLUTION OF CANCER CELL POPULATIONS FOUND IN RNA
Pre-tratamiento Final del tratamiento Post tratamiento
Chemotherapy Drug holiday
time
# of
SN
Vs/
gene
e
xpre
ssio
n
com
plex
ity
cancer cell population RNA heterogeneity
Lopez-Diaz*, Lee* et al. Proc. Natl Acad of Sciences 2014
NEXT: SNP DETECTION IN RNA PLATFORM TO DESIGN NOVEL CONCEPT DRUG SCREENS
S a l k I n s t i t u t e San Diego - USA
N a t i o n a l U n i v e r s i t y o f L i t o r a l Santa Fe - Argentina
Muchas gracias!
Thanks!
Contact: [email protected] [email protected] www.salk.edu www.cepimp.com